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Intrigued by the discoveries that mTORC1 and AMPK share the common activator, v-ATPase-Ragulator complex (Zhang et al., 2014), and that metformin may directly act on the lysosomal pathway to promote AMPK activation (Zhang et al., 2016), we sought to set up a pure in vitro reconstitution system to explore the mechanism of metformin's action ().We stably expressed LAMP1-RFP-FLAG in HEK293T. Metformin Tablets. from. Metformin is a medicine that is used to treat type 2 diabetes by helping to bring your blood sugar levels under control. Metformin is prescribed when lifestyle changes like diet and exercise plans have not been effective in controlling your blood sugars. Treatment for: Type 2 Diabetes. Type of drug: biguanide. Glucophage is a medicine for diabetes oral is used to treat type 2 of diabetes. Its active substance is Metformin which belongs to the class of biguanide substances. 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